The purpose of this study was to evaluate the effect of tetracycline-treated a-PTFE membrane on the human gingival fibroblast attachment. To evaluate the cell attachment to membrane, the number of gingival fibroblast attached to a-PTFE membrane was counted by hemocytometer. Also the cytotoxic effect of tetracycline-treated a-PTFE membrane on human gingival fibroblast was meawred by Wataha´s MTT assay. After tetracycline-treated barrier membrane was exposed to culture media for 24 hours, cell attaachment to membrane and cytotoxic ´effect were measured.
The results were as follows;
As for cell proliferation rate, total cell number increase in both control group and tetracycline-treated a-PTFE membrane in course of time, but the cell numbers were decreased to half in tetracycline-treated barrier membrane as in the control group.
In MTT assay, tetracycline-treated e-PTFE membrane showed moderate to weak cytotoxic effect compared to non-treated control. At the begining, tetracycline-treated, so was barrier membrane released abundant tetracycline and so decreased the number of cell attached barrier membrane, but not almost observed cellular activity inhibition on attached cell and as decreasing tetracycline-treated barrier membrane, the number of attached cell were increased.
These results might suggest that tetracycline-treated a-PTFE membrane showed relatively low proliferation rate of attached cells, but weak cytotoxic effect. So it is suggested tetracycline-treated r.-PTFE membrane could bind gingival connective tissue normally and the healing site could gain stability.
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